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Analytical Techniques in Plant Sciences: Botany for B.Sc. Students | Semester I: Core II

Analytical Techniques in Plant Sciences: Botany for B.Sc. Students | Semester I: Core II

Author : B P Pandey

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  • ISBN : 9789369585502
  • Pages : 248
  • Binding : Paperback
  • Language : English
  • Imprint : S Chand Publishing
  • © year : 2026
  • Size : 6.50*9.25

Price : 299.00 239.20

Quantity : - +

This textbook has been designed to meet the requirements of B.Sc. First Semester Core II Botany students for all Odisha State Universities and Colleges under the recommended National Education Policy 2020. It comprehensively covers theory and practical papers, namely, Analytical Techniques in Plant Sciences.

The theory part of the text introduces the students to the understanding of the microscopy and knowledge to analyse plant samples using electron microscopy and flow Cytometer. The students will learn the use of radioisotopes for analysis of biological samples, chromatographic techniques for separation of amino acids, pigments and biomolecules. The book will help students learn the method for characterising protein and nucleic acids and skill on handling electrophoresis equipment for preparation of gels. Practical part has been presented systematically to help students achieve sound conceptual understanding and learn experimental procedures.

  • Well-labelled diagrams relevant to the topics have been provided for easy understanding of the concepts
  • Engaging chapter-end exercises have been provided for practice
  • Collection of experiments to stimulate interest and aid in learning and memorising the practical aspect of the subject

Theory

Chapter 1: Microscopic Techniques
Chapter 2: Electron Microscopy
Chapter 3: Flow Cytometry
Chapter 4: Centrifugation
Chapter 5: Radioisotopes in Biology
Chapter 6: UV - Vis Spectrometry
Chapter 7: Infrared Spectroscopy
Chapter 8: Fluorescence Spectroscopy
Chapter 9: Other Spectroscopic Methods
Chapter 10: Chromatography
Chapter 11: Gel Electrophoresis
Chapter 12: Mass Spectrometry
Chapter 13: X-Ray Diffraction and X-Ray
Chapter 14: Cell Cycle
Chapter 15: Mitosis and Meiosis

Practicals

Experiment 1: Study of Different Microscopic Techniques Observation through Simple and Compound Microscope
Experiment 2: Study of PCR Using Demonstration
Experiment 3: To Separate Pigments by Paper Chromatography
Experiment 4: To Separate Phytochemicals by Thin Layer Chromatography
Experiment 5: Qualitative Analysis of Biomolecules
Experiment 6: Demonstrations on Scanning Electron Microscopy, Electrophoresis and Chromatography (HPLC)
Experiment 7: Measuring OD Using Spectrometer
Experiment 8: Validation of Beer-Lambert Law

Viva Voce

B.Sc. Botany Students

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